Researchers Develop New Method to Quickly Diagnose Bean Viruses

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[Courtesy of Gyeonggido Business & Science Accelerator]

SEOUL – Viral diseases in soybeans lead to reduced yields and lower quality. Polymerase chain reaction (PCR), a method used in molecular biology, has been widely used to diagnose bean viruses, but it takes up to two weeks to get results. South Korean researchers have developed a screening method for envelope proteins that can easily identify bean viruses.

The common mosaic virus is seed-borne and causes mosaicism and lesions on foliage as well as blackened roots. Yellow mosaic virus is often associated with the presence of virus source plants, with symptoms consisting of a leaf mosaic formed by areas of contrasting yellow or green mosaic.

After placing a sample taken by scraping the skin of a bean into the diagnostic kit, it will be possible to verify the results in five minutes. It enabled the selection of candidate mini-antibodies without a protein purification process, the Gyeonggido Business & Science Accelerator (GBSA) biocenter said, adding that commercialization of diagnostic devices based on GBSA’s technology would replace imports.

The center said its research team had proposed a new platform for the production of plant virus diagnostic kits using the combined module of SpyTag (peptide) and SpyCatcher (protein) for binding of antigens and proteins. envelope. Using SpyTag and SpyCatcher, bioconjugation can be achieved between two recombinant proteins that would otherwise be restrictive or impossible with traditional direct genetic fusion between the two proteins.

“Through continued research and development, it has become possible to diagnose the virus at an early stage and prevent it from being blocked,” an anonymous GBSA official said in a statement. “We will contribute to stable agricultural production by reducing economic losses caused by damage caused by crop viruses.” The study was conducted jointly with researchers from Kyungpook National University, Rural Development Administration and Ajou University College of Medicine.

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